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Sains Malaysiana 53(11)(2024): 3761-3770
http://doi.org/10.17576/jsm-2024-5311-18
Pemencilan, Pencirian dan Afiliasi Filogenetik Endofit Streptomyces sp. Bioaktif daripada Pokok yang Mempunyai Nilai Perubatan
(Isolation, Characterisation and
Phylogenetic Affiliation of Bioactive Endophytic Streptomyces sp.
Associated with Medicinal
Plants)
NURUL
‘IZZAH MOHD SARMIN1,2 & NORAZIAH MOHAMAD ZIN3,*
1Centre of Preclinical Science Studies, Universiti Teknologi MARA, Sungai Buloh Campus, Jalan Hospital, 47000 Selangor,
Malaysia
Received: 29 August
2023/Accepted: 6 September 2024
Abstrak
Kepelbagaian biologi tumbuhan di hutan Malaysia menyediakan persekitaran yang sesuai untuk pemencilan Streptomyces endofit. Strain novel Streptomyces merupakan sumber berpotensi dalam menghasilkan sebatian farmaseutikal bioaktif yang boleh dibangunkan sebagai ubatan baharu. Kajian ini bertujuan untuk memencilkan Streptomyces endofit daripada tumbuhan ubatan yang berbeza di Hutan Simpan Bangi, pengenalpastian, pencirian dan analisis filogenetik pencilan tersebut. Streptomyces endofit dipencilkan menggunakan kaedah sterilisasi permukaan dan pencilan ini seterusnya dikenal pasti menggunakan pemerhatian morfologi. Streptomyces endofit yang dipencilkan telah dikelaskan ke dalam kumpulan berdasarkan warna miselium aerial. Analisis jujukan gen 16S rRNA telah dijalankan untuk mentakrifkan hubungan filogenetik antara spesies yang berkait rapat dan juga antara strain yang tergolong dalam satu spesies. Semua pencilan kemudiannya diuji untuk aktiviti antimikrob. Tiga Streptomyces endofit iaitu SUK 8, SUK 10
dan SUK 15 telah berjaya dipencilkan daripada tumbuhan ubatan yang berbeza. Ketiga-tiga pencilan tersebut dikelaskan dalam kumpulan siri kelabu berdasarkan warna miselium aerial. Siri kelabu ini juga membentuk kelompok filogenetik yang sama berdasarkan data jujukan gen 16S rRNA. Berdasarkan analisis filogenetik jujukan gen 16S rRNA, semua pencilan dikelaskan sebagai Streptomyces eurythermus ATCC 14975T (persamaan jujukan gen 98.5%). Pencilan SUK 8, SUK 10 dan SUK 15 juga menunjukkan corak aktiviti biologi yang serupa yang berupaya merencat sehingga 100% organisma patogen yang sama. Keputusan ini mengesahkan bahawa terdapat korelasi yang baik antara kepelbagaian fenotip antimikrob dan gen
16S rRNA. Kesimpulannya, metabolit aktif yang berpotensi daripada Streptomyces endofit boleh dijangka daripada data taksonomi yang baik.
Kata kunci: Endofit; kajian filogenetik; Streptomyces; tumbuhan ubatan; 16S Rrna
Abstract
The high
biodiversity of plant species in Malaysian forests provides a suitable
environment for the isolation of endophytic Streptomyces. Novel strains
of Streptomyces are potential sources for producing bioactive
pharmaceutical compounds that can be developed as new drug candidates. This
study aims to isolate the endophytic Streptomyces from different
medicinal plants in the Bangi Reserve Forest before
identification, characterisation, and phylogenetic analysis of the isolates.
The endophytic actinomycetes were isolated using surface-sterilization method
and further identified through morphological observation. The isolated endophytic Streptomyces were classified based on the colour of aerial mycelium. 16S rRNA gene sequence
analysis was done to define the phylogenetic relationships among closely
related species and strains belonging to a species. All isolates were then
tested for their antimicrobial activities. Three endophytic Streptomyces,
SUK 8, SUK 10 and SUK 15 were successfully isolated from different medicinal
plants. These three isolates were classified into a grey series group based on
the colour of aerial mycelium. The grey series also formed the same phylogenetic
clade based on the 16S rRNA sequence data. Phylogenetic analysis of 16S rRNA
gene sequences showed all three isolates were classified as Streptomyces eurythermus ATCC 14975T (gene sequence
similarity 98.5%). Isolates of SUK 8, SUK 10 and SUK 15 also showed similar
patterns of biological activities with inhibition of up to 100% of the same
pathogenic organisms. These findings
proved a good correlation between the diversity of the antimicrobial phenotype
and the 16S rRNA gene. In conclusion, the potential of active metabolites from
endophytic Streptomyces can be expected from a good
source of taxonomy data.
Keywords:
Endophytes; medicinal plants; phylogenetic study; Streptomyces; 16S rRNA
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